Methods in Yeast Genetics and Genomics: A Cold Spring Harbor Laboratory Course Manual, 2015 Edition

"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor Laboratory for the last forty years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents u [...]
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"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor Laboratory for the last forty years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2005), revolutionary advances in genomics and proteomics technologies have had a significant impact on the field. The 11 experiments included in this manual provide a foundation of methods for any modern-day yeast lab. These methods emphasize combinations of classical and modern genetic approaches, including isolation and characterization of mutants, two-hybrid analysis, tetrad analysis, and complementation and recombination; and molecular genetic techniques are also covered. Additional experiments introduce fundamental techniques in yeast genomics, including both performance and interpretation of multiplexed sequencing and comparative genome hybridization to DNA arrays. Comparative genomics is introduced using different yeast strains to study natural variation, evolution, and quantitative traits. This manual covers the full repertoire of genetic approaches needed to dissect complex problems in the yeast Saccharomyces cerevisiae.